Estari Mamidala
Kakatiya University, India
Title: Potent anti-human immunodeficiency virus activity of 4',7-dihydroxy-3-methoxy- 5,6-dimethylflavone, a novel compound blocking HIV-1 reverse transcriptase
Biography
Biography: Estari Mamidala
Abstract
Introduction: HIV is a major global concern due to its drug resistance and warrants the development of novel antivirals as an alternative therapy. This study aims to test the anti-HIV activity of a novel compound 4',7-dihydroxy-3-methoxy-5,6-dimethylflavone, isolated from Hydrastis canadensis.
Methods: The crude extracts were prepared from dried areal parts Hydrastis canadensis in methanol by Soxhlet method and isolated 4',7-dihydroxy-3-methoxy-5,6-dimethylflavone by using column chromatography and HPLC. Peripheral Blood Mononuclear Cells (PBMCs) isolated from healthy donors by Ficoll-Hypaque density gradient centrifugation method. Cell viability test was performed on 4',7-dihydroxy-3-methoxy-5,6- dimethylflavone by MTT assay using PBMC and HIV-1 RT inhibition activity was determined by HIV-1 Reverse Transcriptase (p66) Capture ELISA.
Results: In the HIV-1 reverse transcriptase assay, isolated compound showed good inhibitory activity (92%) at 110 μg/ml and MTT assay confirmed that the isolated 4',7-dihydroxy-3-methoxy-5,6-dimethylflavone had no cytotoxic activity and IC50 values higher than 100 μg/ml.
Conclusion: The compound isolated from Hydrastis canadensis seemed to have the potential to act as a source of useful drugs and also to improve the health status of the consumers as a result of the presence of active compound that are vital for good health.